L Carnitine is Underrated. New Meta Review Reminds Us Why. Metabolism: The carnitine shuttle and fatty acid metabolism (practice) Khan Academy Carnitine transport and fatty acid oxidation ScienceDirect Short Note On Carnitine Shuttle: Fatty acids are oxidized in mitochondria, and synthesized in the cytoplasm. Long chain fatty acids must form an active intermediate, fatty acyl CoA, before being oxidized inside mitochondria. Catherine Fatty acid transportation mechanism in cell, starting with the activation of fatty acid by carnitine palmitoyl transferase 1 to acyl coA, which now attaches itself with the translocase to be transported across