Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol's Impact on Glutathione and Liver Damage Liver specific Bcl3 Knockout Alleviates Acetaminophen induced Liver Injury by Activating Nrf2 Pathway in Male Mice Cellular and Molecular Gastroenterology and Hepatology Unraveling the effect of intra and intercellular processes on acetaminophen induced liver injury npj Systems Biology and Applications TUSKC Japanese Glutathione Tablets for Skin Brightening with Vitamin C Lifezen Healthcare