Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. How Keeping Glutathione Levels Up Can Benefit Your Health & Your Vitamin D Status GrassrootsHealth Glutathione Deficiency Symptoms: Is Poor Sleep Just the Beginning? Cardiology & Neurology Specialists located in Queens, Forest Hills and Brighton Beach, Brooklyn, NY Advanced Medical Care Glutathione depletion and dual model oxygen balance disruption for photodynamic therapy enhancement ScienceDirect Identification of cytotoxic, glutathione reactive moieties inducing accumulation of reactive oxygen species via glutathione depletion ScienceDirect