Since Tylenol is such a hot topic right now, did you know it depletes glutathione levels? When your body processes Tylenol in the liver, one of the pathways creates a toxic byproduct. To neutralize Acetaminophen Toxicity: How Genetic Susceptibility Combines with Environmental Factors Acetaminophen or Tylenol Toxicity Lect 19 Pharmacology View of Acetaminophen, the missing anion gap in the patient with metabolic acidosis The Southwest journal of medicine Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.